The 30-share barometer, which had gained 33.40 points in Tuesday's volatile session, moved down by 16.29 points, or 0.06 percent, to 26,614.22, led by weakness in banking, oil & gas and FMCG sector stocks.
Similarly, the National Stock Exchange index Nifty fell 8.05 points, or 0.10 percent, to 7,956.75.
Brokers said apart from profit-booking by speculators after Tuesday's gains, capital outflows by foreign funds and a mixed trend in overseas markets, influenced sentiments here.
Besides, cautious approach by participants in view of a holiday-shortened week as markets will remain closed on Thursday and Friday for Mahatma Gandhi Jayanthi and Dussehra, respectively, also cast its shadow, they said.
Among other Asian bourses, Japan's Nikkei was trading 0.26 percent higher in morning trade, while Hong Kong's financial markets are closed for public holiday.
US Dow Jones Industrial Average ended 0.17 per cent lower in Tuesday's trade.