The 30-share barometer, which had gained 6.58 points in Wednesday's choppy trade, moved down by 77.78 points, or 0.32 percent, to 24,478.31 with IT, auto, PSUs, metal and banking stocks leading the fall.
Also, the National Stock Exchange index Nifty shed 13.25 points, or 0.18 percent, to 7,316.40.
Brokers said that offloading of positions by foreign funds and retail investors on the last session of May's expiry in the derivatives segment and mixed cues from other Asian markets influenced the trading sentiments.
Shares of Infosys plunged 6.10 percent to Rs 2,978.75 after the company on Wednesday announced the resignation of board member and president BG Srinivas who was considered among the top contenders for the first non-founder CEO post.
Among other Asian markets, Japan's Nikkei fell 0.58 percent, while Hong Kong's Hang Seng was trading 0.22 percent higher in morning trade.
US Dow Jones Industrial Average ended 0.25 percent down in Wednesday's trade.


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